Scientific Studies

Many recent studies have shown promising findings in the field of regenerative medicine.  In our ongoing efforts to encourage participation and collaboration from all areas of medicine, Celling Technologies will continue to compile and update this highly informative and educational information for public review.

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• Bret M. Schipper, MD, et al, Regional Anatomic and Age Effects on Cell Function of Human Adipose-Derived Stem Cells, Annals of Plastic Surgery 2008 Vol. 60 #5:538-544.
• M. Knippenberg, et al, Osteogenesis versus chondrogenesis by BMP-2 and BMP-7 in adipose stem cells, Biochemical and Biophysical Research Communications 342 (2006) 902–908.
• Lauren S. Sefcik, et al, Collagen nanofibres are a biomimetic substrate for the serum-free osteogenic differentiation of human adipose stem cells, J Tissue Eng Regen Med 2008; 2: 210–220.
• Neil H Riordan, et al, Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis, Journal of Translational Medicine 2009, 7:29.
• Mandi J. Lopez, et al, Acceleration of Spinal Fusion Using Syngeneic and Allogeneic Adult Adipose Derived Stem Cells in a Rat Model, Journal of Orthopaedic Research 2008.
• Arnaud Scherberich, et al, Three-Dimensional Perfusion Culture of Human Adipose Tissue-Derived Endothelial and Osteoblastic Progenitors Generates Osteogenic Constructs with Intrinsic Vascularization Capacity, STEMCELLS 2007;25:1823–1829.
• Jeong-Min Kim, et al, Systemic transplantation of human adipose stem cells attenuated cerebral inflammation and degeneration in a hemorrhagic stroke model, Brain Research 1183 (2007) 43–50.
• De Ugarte, D.A., et al, Comparison of Multi-Lineage Cells from Human Adispose Tissue and Bone Marrow, Cells Tissues Organs 2003 174:101-109.
• Fraser, J.K.,et al, Fat tissue: an underappreciated source of stem cells for biotechnology, Trends in Biotechnology 2006.
• Gomillion, C.T., et al, Stem Cells and Adipose Tissue Engineering, Science Direct, March, 2006.
• Hattori, H., et al, Bone Formation Using Human Adipose Tissue-Derived Stromal Cells and Biodegradable Scaffold, Wiley InterScience, July 2005.
• Hattori, H., et al, Osteogenic Potential of Human Adipose Tissue Derived Stromal Cells as an Alternative Stem Cell Source, Cells Tissues Organs 2004.
• Helder,M.N., et al, Stem Cells from Adipose Tissue Allow Challenging New Concepts for Renerative Medicine, Tissue Engineering~ Submitted 2007.
• Hoogendoorn, et al, Adipose stem cells for intervertebral disc regeneration: current status and concepts for the future, Journal of Cellular and Molecular Medicine ~ February 2008.
• Hsu, J., Wang, J., Liu, N., Krenek, L., Zuk, P., Hedrick, M., Benhaim, P., Lieberman, J., et al, Stem Cells from Human Fat as Cellular Delivery Vehicles in a Rat Posterolateral Spine Fusion Model. The Spine Journal, Volume 7, Issue 5, Pages 7S-7S.
• Jagur-Grodzinski,J., et al, Polymers for Tissue Engineering, Medical Devices and Regenerative Medicine, Wiley InterScience 2006.
• Kern, S., et al, Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood or Adipose Tissue, Stem Cells, Jan., 2006.
• Lendeckel, S., et al Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: case report, Journal of Cranio-Maxillofacial Surgery, June 2004.
• Mitchell, J.B., et al, Immunophenotype of Human Adipose-Derived Cells: Temporal Changes in Stromal-Associated and Stem Cells, Stem Cells 2006;24:376-385.
• Mao, J.J., et al, Craniofacial Tissue Engineering by Stem Cells, J Dent Res 85(11) 2006.
• Niechoda, B., et al, In vivo bone formation using Adipose Derived Stromal Stem Cells, The histomorphometry of the ovine defect model, Thesis, University of New South Wales 2006.
• Parker, A.M., & Katz, A.J., Adipose-derived stem cells for the regeneration of damaged tissues, Expert Opinion Biological Therapy (2006)6(6).
• Rodriguez, L.V., et al, Clonogenic multipotent stem cells in human adipose tissue differenciate into functional smooth muscle, Proceeding of the National Academy of Sciences Aug 8th, 2006, Vol. 103 no.32 12167-12172.
• Schaeffler, A., et al, Concise Review: Adipose Tissue-Derived Stromal Cells - Basic and Clinical Implications for Novel Cell-Based Therapies. Stem Cells, 2007.
• Sheyn, D., et al, Nonvirally Engineered Porcine Adipose Tissue-Derived Stem Cells: Use in Posterior Spinal Fusion. Stem Cells, Vol. 26, 1056-1064, 2008.
• Strem, S.S., et al, Multipotential differenciation of adipose tissue-derived stem cells, Keio J Med 54 2005(3)132-141.
• Tobita, M., et al, Periodontal Tissue Regeneration with Adipose-Derived Stem Cells, Tissue Engineering, Volume 00, Number 00, 2007.
• Zuk, Patricia A., et al, Human Adipose Tissue is a Source of Multipotent Stem Cells, Molecular Biology of the Cell, December, 2002.

Biomechanical Regenerative Cell Stimulation - Top

• Beck, B., et al, Novel, high-frequency, low-strain mechanical loading for premenopausal women with low bone mass: early findings, Journal of Bone and Mineral Research, Vol. 24, 505-507, 2004.
• Bonewald, L., Osteocytes as multifunctional cells , Journal of Musculoskeletal and Neuronal Interactions, Vol. 6, 331-333, 2006.
• Cardinale, M., et al, Gastrocnemius Medialis and Vastus Lateralis Oxygenation during Whole-Body Vibration Exercise, Official Journal of the American College of Sports Medicine, Vol. 39, 694-700, 2007.
• Cardinale, M., et al, Hormonal responses to a single session of whole body vibration exercise in elderly individuals, British Journal of Sports Medicine, Online, 15 April 1008.
• Flieger, J., et al, Mechanical Stimulation in the Form of Vibration Prevents Postmenopausal Bone Loss in Ovariectomized Rats, Calcified Tissue International, Vol. 63, 510-514,1998.
• Gusi, N., et al, Low-frequency vibratory exercise reduces the risk of bone fracture more than walking: a randomized controlled trial , BMC Musculoskeletal Disorders, Vol. 7, 92, 2006.
• Hannan, M., et al, Establishing the compliance in elderly women for use of a low level mechanical stress device in a clinical osteoporosis study, Osteoporosis International, Vol. 15, 918-928, 2004.
• Humphries, B., et al, The assessment of vibration signals during a fatigue protocol, The School of Health and Human Performance, Central Queensland University.
• Jordan, M., et al, Vibration Training: An Overview of the Area, Journal of Strength and Conditioning Research, Vol. 19, 459-466, 2005.
• Jordon, J., Good vibrations and strong bones? , AJP: Regulatory, Integrative, and Comparative Physiology, Vol. 288, 555-556, 2004. .
• Karachalios, L., et al, Mechanical Stimulation in the Form of Vibration Prevents Postmenopausal Bone Loss in Ovariectomized Rats , Laboratory for Research of the Musculoskeletal System, Vol. 63, 510-514, 1998.
• Kremmler, W., et al, Benefits of 2 Years of Intense Exercise on Bone Density, Physical Fitness, and Blood Lipids in Early Postmenopausal Osteopenic Women , Archives of Internal Medicine, Vol. 164, 2004.
• Rubin, C., et al, The Use of Low-Intensity Ultrasound to Accelerate the Healing of Fractures , The Journal of Bone and Joint Surgery, Vol. 83-A, 259-270, 2001.
• Rubinacci, A., et al, Ovariectomy Sensitizes Rat Cortical Bone to Whole-Body Vibration, Calcified Tissue International, Vol. 83, 316-326, 2008.
• Russo, C., et al, High-Frequency Vibration Training Increases Muscle Power in Postmenopausal Women , Archives of Physical Medicine and Rehabilitation, Vol. 84, 1854-1857, 2003.
• Semler, O., et al, Preliminary results on the mobility after whole body vibration in immobilized children and adolescents , Journal of Musculoskeletal and Neuronal Interactions, Vol. 7, 77-81, 2007.
• Spencer, G., et al, Long-term potentiation in bone - a role for glutamate in strain-induced cellular memory? , BMC Stem Cell Biology, Vol. 4, 1-4, 2003.
• Torvinen, S., et al, Effect of four-month vertical whole body vibration on performance and balance, Medicine and Science in Sports and Exercise, Vol.34.9, 1523-1528, 2002.
• Turner, C., et al, Exercise As An Anabolic Stimulus For Bone, Current Pharmaceutical Design, Vol. 10.21, 2629-2641, 2004.
• Verschueren, S., et al, Effect of 6-Month Whole Body Vibration Training on Hip Density, Muscle Strength, and Postural Control in Postmenopausal Women: A Randomized Controlled Pilot Study , Journal of Bone and Mineral Research, Vol. 29, Number 3, 2004.
• Warman, G., et al, The influence of vibration on maximal torque generation, The School of Health and Human Performance , Central Queensland University.
• Yang, Y., et al, Enhancement of Mechanical Signals for Tissue Engineering Bone , Topics in Tissue Engineering, Vol. 2, 2005.

Bone Marrow Derived Stem Cells - Top

• Centeno, Christopher, et al, Regeneration of meniscus cartilage in a knee treated with percutaneously implanted autologous mesenchymal stem cells, Medical Hypotheses (2008) 71, 900–908.
• Johnson, Robert, et al, Concentrated Autologous Adult Stem Cells vs. Autologous Iliac Crest Bone for Instrumented Fusion of the Lumbar Spine: Clinical and Laboratory Results for Two Consecutive Patients , Neurosurgical Associates 4410 Medical Dr., San Antonio, TX 78229 Tel: 210-614-2453 November 17, 2008.
• Gan, Yaokai, et al, The Clinical Use of Enriched Bone Marrow Stem Cells Combined with Porous Beta-tricalcium Phosphate in Posterior Spinal Fusion, Biomaterials; 29 (2008) 3973–3982.
• Connolly, J., et al, Development of an Osteogenic Bone Marrow Preparation , The Journal of Bone & Joint Surgery Volume 1989 ; 71 684-691.
• Gangji,V and Hauzeur, J.P.,et al, Treatment of Osteonecrosis of the Femoral Head With Implantation of Autologous Bone Marrow Cells, The Journal of Bone & Joint Surgery Volume 87-A Supplement 1, Part 1, March 2005.
• Gangji,V, et al, Treatment of Osteonecrosis of the Femoral Head With Implantation of Autologous Bone Marrow Cells-A PILOT STUDY, The Journal of Bone & Joint Surgery Volume 86-A, Number 6, June 2004.
• Gupta, Anu R., et al, Perioperative and Long-Term Complications of Iliac Crest Bone Graft Harvesting for Spinal Surgery: A Quantitative Review of the Literature, International Medical Journal Vol. 8, Number 3, pp 163-166, September 2001. .
• Hernigou, P. et al, The use of percutaneous autologous bone marrow transplantation in nonunion and avascular necrosis of bone , The Journal of Bone and Joint Surgery Vol 87-B, Number 7, July 2005.
• Hernigou, P. and Beaujean, F., Treatment of Osteonecrosis With Autologous Bone Marrow Grafting, Clinical Orthopaedics and Related Research Number 405, pp 14-23 December 4, 2002.
• Hernigou, P. and Beaujean, F., Percutaneous Autologous Bone Marrow Grafting For Nonunions, The Journal of Bone & Joint Surgery Vol 87-A, Number 7, Page 1430-1437 July 2005.
• Johnson, R., et al, Concentrated From Bone Marrow Aspirate Mixed With Crushed Cancellous Bone Chips- A Substitute for Autnograft Fusion in the Spine? .
• Kevy, S.V., et al, Point of Care Concentration of Bone Marrow , Children's Hospital, Harvard Medical School, October 2006.
• McLain, R.F., et al, Aspiration of Osteoprogenitor Cells of Augmenting Spinal Fusion: Comparison of Progenitor Cell Concentrations from the Vertebral Body and Illiac Crest , The Journal of Bone & Joint Surgery Vol 87-A, Number 12, Page 2655-2661 December 2005.
• Muschler, G.F., et al, Spine Fusion Using Cell Matrix Composites Enriched in Bone Marrow-Derived Cells , Clinical Orthopaedics and Related Research Number 407 pp 102-118.
• Schultz, S.S., et al, Adult Stem Cell Application in Spinal Cord Injury, Current Drug Targets, 2005, 6, 63-73.
• Yoon et al, Complete Spinal Cord Injury Treatment using Autologous Bone Marrow Cell Transplantaion and Bone Marrow Stimulation with Granulocyte Macrophage-Colony Stimulating Factor 9GM-CSF) Phase I/II Clinical Trial , Stem Cells April 26, 2007.

General Orthopaedics - Top

• Rotini, Roberto et al, New perspectives in rotator cuff tendon regeneration: review of tissue engineered therapies , Chir Organi Mov (2008) 91:87–92
• Zantop, Thore et al, Extracellular Matrix Scaffolds Are Repopulated by Bone Marrow-Derived Cells in a Mouse Model of Achilles Tendon Reconstruction, Journal of Orthopaedic Research June 2006, 1299-1309
• Hernigou, Philippe et al, Abnormalities in the Bone Marrow of the Iliac Crest in Patients Who Have Osteonecrosis Secondary to Corticosteroid Therapy or Alcohol Abuse , J Bone Joint Surg Am. 1997;79:1047-53.
• Centeno, Christopher, et al, Regeneration of meniscus cartilage in a knee treated with percutaneously implanted autologous mesenchymal stem cells, Medical Hypotheses (2008) 71, 900–908.
• Chen, Faye, et al, Technology Insight: adult stem cells in cartilage regeneration and tissue engineering, NATURE CLINICAL PRACTICE RHEUMATOLOGY, JULY 2006 VOL 2 NO 7, 373-382.
• Acosta F., Lotz J., Ames C., et al, The potential role of mesenchymal stem cell therapy for intervertebral disc degeneration: a critical overview , Neurosurg Focus 19 (3):E4, 2005.

General Stem Cell - Top

• Bonnet, D., Biology of human bone marrow stem cells. Clin Exp Med Vol. 3, 140-149, 2003.
• Cipolleschi, M., et al, The Role of Hypoxia in the Maintenance of Hematopoietic Stem Cells , Blood, Vol. 82, 2031-2037, 1993.
• Daley, G. Q., Prospects for stem cell therapeutics- myths and medicines, Current Opinion on Genetic Development, Vol. 12, 607-613, 2002.
• Eiges, R., et al, A molecular view on pluripotent stem cells . FEBS Letters, Vol. 529, 135-141, 2002.
• Ema, H., et al, Self-renewal and lineage restriction of hematopoietic stem cells. Current Opinion on Genetic Development, Vol. 13, 508-512, 2003.
• Fuchs, E., Tumbar, T., and Guasch, G. Socializing with the Neighbors: Stem Cells and Their Niche . Cell Vol. 116 , 769-778, 2004. .
• Gafni, Y., et al, Stem cells as vehicles for orthopedic gene therapy , Gene Therapy, Vol. 11, 417-426, 2004.
• Herzog, E. L., et al, Plasticity of marrow-derived stem cells. Blood , Vol. 102, 3483-3493, 2003.
• Kondo, M., et al, Biology of Hematopoietic Stem Cells and Progenitors: Implications for Clinical Application, Annual Review of Immunology, Vol.21, 759-806, 2003.
• Risbud Jefferson Medical College, et al, Cellular Therapy for Disc Degeneration, SPINE Volume 30, Number 17S, pp S14–S19.
• Shen, Francis H., et al, Cell Technologies for Spinal Fusion, The Spine Journal Volume 5 231s-239s, 2005.
• Szilvassy SJ, The Biology of Hematopoietic Stem Cells, Archives of Medical Research, 2003 Vol. 34, 446-460.
• Taichman, S, Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche , Blood, Vol. 105, 2631-2639, 2005.
• Tepper, O, et al, Adult vasculogenesis occurs through in situ recruitment, proliferation, and tubulization of circulating bone arrow-derived cells , Blood, 2005; Vol. 95, 1068-1077, 2005.
• ABC A Dog's Stem Cell Life .
• Understanding Stem Cells .
• National Institutes of Health, Regenerative Medicine 2006 NIH .

Genetically Modified Stem Cells - Top

• Morizono, K.; et al, Multilineage Cells from Adipose Tissue as Gene Delivery Vehicles, Human Gene Therapy, Vol.14, 59-66, 2003.
• Peterson, B., et al, Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue, Tissue Engineering, Vol. 11, 120-9, 2005.
• Riew, K. D., et al, Thoracoscopic intradiscal spine fusion using a minimally invasive gene-therapy technique, Journal of Bone and Joint Surgery, Vol. 85, 866-71, 2003.
• Vaccaro, A. R., et al, A 2-year follow-up pilot study evaluating the safety and efficacy of op-1 putty (rhbmp-7) as an adjunct to iliac.pdf.
• Viggeswarapu, M. et al, Adenoviral delivery of LIM mineralization protein-1 induces new-bone formation in vitro and in vivo , Journal of Bone and Joint Surgery, Vol. 83, 364-76, 2001.
• Wang, J. C., et al, Effect of regional gene therapy with bone morphogenetic protein-2-producing bone marrow cells on spinal fusion in rats, Journal of Bone and Joint Surgery, 85-A(5)- 905-11, 2005.
• West, J. L., et al, Results of spinal arthrodesis with pedicle screw-plate fixation , Journal of Bone and Joint Surgery, Vol. 73(8) - 1179-84, 1991.
• Yang, M., et al, In vitro and in vivo induction of bone formation based on ex vivo gene therapy using rat adipose-derived adult stem cells expressing BMP-7. Cytotherapy, Vol. 7(3), 273-81, 2005.
• Yoon, E., In Vivo Osteogenic Potential of Human Adipose-Derived Stem Cells/Poly Lactide-Co-Glycolic Acid Constructs for Bone Regeneration in a Rat Critical-Sized Calvarial Defect Model .

Donor Site Morbidity - Top

• Coventry, M. B., and Tapper, E. M., Pelvic Instability: A Consequence of Removing Iliac Bone for Grafting , The Journal of Joint and Bone Surgery, Vol. 54, 83-101, 1972.
• Cowley, S. P., and Anderson, L. D., Hernias Through Donor Sites for Iliac-Bone Grafts , The Journal of Joint and Bone Surgery. Vol. 65, 1023-5, 1983.
• Reid, R., Hernia Through an Iliac Bone-graft Donor Site: A Case Report , The Journal of Joint and Bone Surgery, Vol. 50, 757.
• Romih, M., et al, The Vertibral Interbody Grafting Site's Low Concentration in Osteogenic Progenitors can Greatly Benefit from Addition of Iliac Crest Bone Marrow, European Spine Journal, Vol. 14, 645-648, 2005.

Knee - Top

• Centeno, Christopher, et al, Regeneration of meniscus cartilage in a knee treated with percutaneously implanted autologous mesenchymal stem cells, Medical Hypotheses (2008) 71, 900–908.
• Hicok, K. C., et al, Human adipose-derived adult stem cells produce osteoid in vivo, Tissue Engineering, Vol. 10(3-4), 371-80, 2004.
• Javazon, E., Mesenchymal stem cells paradoxes of passaging , Expiremental Hematology, 32(5)- 414-25, 2004.
• Kraus, K., Critically sized osteo-periosteal femoral defects: a dog model, Journal of Investigative Surgery, Vol. 12(2), 115-24, 1999.
• Loening, A. M., et al, AMIDE: A Free Software Tool for Multimodality Medical Image Analysis, Molecular Imaging, Vol. 2(3), 131-7, 2003.

PRP - Top

• de Boer, H., et al, Fibrin and Activated Platelets Cooperatively Guide Stem Cells to a Vascular Injury and Promote Differentiation Towards an Endothelial Cell Phenotype, Arteriosclerosis, Thrombosis, and Vascular Biologogy, Vol., 26,1653-1659, 2006.
• Massberg, S., et al, Platelets secrete stromal cell-derived factor 1 and recruit bone marrow-derived progenitor cells to arterial thrombi in vivo, Journal of Experimental Medicine, Vol. 203, 1221-1233, 2006.

Shoulder - Top

• Kovacevic BS, David et al, Biological Augmentation of Rotator Cuff Tendon Repair, Clin Orthop Relat Res (2008) 466:622–633

Spine - Top

• Nakajima MD, Takashi, et al, Evaluation of Posterolateral Spinal Fusion Using Mesenchymal Stem Cells, SPINE Volume 32, Number 22, pp 2432–2436
• Fu, Tsai-Sheng, et al, Enhancement of Posterolateral Lumbar Spine Fusion Using Low-Dose rhBMP-2 and Cultured Marrow Stromal Cells, Journal of Orthopaedic Research March 2009, 380-4
• Acosta F., Lotz J., Ames C., et al, The potential role of mesenchymal stem cell therapy for intervertebral disc degeneration: a critical overview, Neurosurg Focus 19 (3):E4, 2005.
• Baksh D., et al, Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy, J. Cell. Mol. Med. Vol 8, No 3, 2004 pp. 301-316.
• Crevesten G., et al, Intervertebral Disc Cell Therapy for Regeneration: Mesenchymal Stem Cell Implantation in Rat Intervertebral Discs, Annals of Biomedical Engineering, Vol. 32, No. 3, March 2004 pp. 430–434.
• Heck J., et al, A Classification System for the Assessment of Lumbar Pain in Athletes, Journal of Athletic Training 2000;35(2):204–211.
• Tow B., et al, Disc Regeneration: A Glimpse of the Future, 2007 The Congress of 128 Neurological Surgeons.