MitoView Fix 640 | 70082

Fluorogenic mitochondrial stains for stay cells that unexpectedly collect in mitochondria and may be imaged with out washing. MitoView 405 is a blue fluorescent mitochondrial dye with absorbance/emission at 398/440 nm, appropriate for detection via way of means of confocal microscopy or glide cytometry the use of settings for DAPI or Pacific Blue. The dye is membrane permeable and will become brightly fluorescent upon accumulation withinside the mitochondrial membrane.

Mitochondrial localization is depending on mitochondrial membrane ability; while membrane ability is disrupted the dye relocalizes to the cytoplasm, however nevertheless keeps fluorescence. The dye is designed to be used in stay cells and isn’t fixable. MitoView 405 is a alternative for our unique Mito View Blue, and has progressed photostability.

MitoView Green

Mito View Green is a inexperienced fluorescent mitochondrial dye with residences just like the ones of Mito Tracker Green FM. The dye is non-fluorescent till it walls into the mitochondrial membrane. The staining is based on mitochondrial mass, now no longer on mitochondria membrane ability. Thus, the dye may be used to stain mitochondria in each stay cells and stuck cells. Mito View Green can be rather membrane ability-structured in yeast cells.

MitoView 633

Mito View 633 is a far-crimson fluorescent mitochondrial dye with absorbance/emission at 622/648 nm.

• The dye is membrane-permeant and will become brightly fluorescent upon accumulation withinside the mitochondrial membrane.
• Staining is depending on mitochondrial membrane ability, and may be used to screen mitochondrial membrane ability in intact cells.
• The dye is designed to be used in stay cells, and isn’t fixable. Note: The most advantageous detection settings for MitoView 633 are similar to for Cy five and different far-crimson dyes.
• However, the dye additionally has seen crimson fluorescence and may be imaged the use of Cythree settings as well.
• As a consequence, it is able to now no longer be viable to apply the dye for -colour imaging with different crimson probes.

MitoView 650

Mito View 650 is a far-crimson fluorescent mitochondrial dye that isn’t depending on mitochondrial membrane ability. The dye has excitation/emission at 644/670 nm for the Cyfive channel. Unlike MitoView 633, it does now no longer bleed into the seen crimson channel, and so may be mixed with crimson probes for multicolor imaging. The dye fluorescence isn’t misplaced after mitochondrial depolarization or fixation, however localization will become non-specific.

MitoView 720

MitoView 720 is a near-infrared mitochondrial dye with absorbance/emission at 720/758 nm. While optimally detected the use of Cy7 settings, the dye is vivid sufficient to be imaged withinside the Cyfive channel, and may be mixed with seen crimson fluorescent probes. Mitochondrial localization is depending on mitochondrial membrane ability; while membrane ability is disrupted the dye relocalizes to the cytoplasm however nevertheless keeps fluorescence.

More Mitochondrial Dyes -In addition to MitoView dyes, Biotium additionally gives numerous classical dyes for measuring mitochondrial membrane ability. These consist of JC-1, a ratiometric dye that paperwork crimson aggregates in healthful cells, which can be decreased to the inexperienced monomeric country while mitochondrial membrane ability is misplaced. We additionally provide TMRM and TMRE, crimson fluorescent dyes which are used to quantitatively degree mitochondrial membrane ability the use of the Nernst equation.